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KMID : 0644120090220020043
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Journal of Oriental Gynecology
2009 Volume.22 No. 2 p.43 ~ p.59
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Inhibitory Effect of Persimmon Leaves on Melanin Synthesis and its Action Mechanism in B16F10 cells
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Jang Du-Hyun
Yoo Dong-Youl
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Abstract
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Purpose: This study was performed to determine the inhibitory effect of Persimmon Leaves extract (PL) on melanin synthesis in B16F10 melanoma cells B16F10.
Methods: The inhibitory effects of PL on melanin synthesis were determined by in vitro assay. To elucidate inhibitory effects of PL on melanin synthesis, we determined the melanin release and melanin production in B16F10. And to investigate the action mechanism, we assessed the gene expression of tyrosinase, TRP-1, TRP-2, PKA, PKC, ERK-1, ERK-2, AKT-1, MITF in B16F10.
Results: 1. PL inhibited melanin release, melanin production in B16F10. 2. PL inhibited tyrosinase activity in vitro and in B16F10. 3. PL suppressed the expression of tyrosinase, TRP-1, TRP-2 in B16F10. 4. PL suppressed the expression of PKA, PKC in B16F10. 5. PL increased the expression of ERK-1, ERK-2, AKT-1 in B16F10. 6. PL suppressed the expression of MITF in B16F10.
Results: From these results, it may be concluded that PL is possesed of the antimelanogenetic effects.
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KEYWORD
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Persimmon Leaves extract (PL), melanin synthesis, antimelanogenetic effect, tyrosinase
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